Methods library · Genes and genomes
Gene targeting: knockout and knock-in mice
Also called knockout mouse, knock-in mouse, homologous recombination in ES cells
Deleting or replacing a gene in the mouse germ line by homologous recombination in embryonic stem cells.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
77 lessons use this method.
Cell Signaling
- Cytokine Receptors and JAK–STAT
- TGF-β and Smads
- Wnt: β-Catenin and Planar Polarity
- Hedgehog and the Primary Cilium
- Innate Immunity: Toll-like Receptors and NF-κB
- Antigen Receptors and Checkpoints
- Ion Channels as Receptors
- Signaling to the Nucleus: CREB, AP-1 and Immediate Early Genes
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Death Receptors, BCL-2 and Caspases
- Long-Distance Signaling in Neurons
- Reverse Signaling: When the Ligand Is Also a Receptor
- Hacking the Cell: Reporters, Actuators and Genetic Tools
Developmental Neuroscience
- Tools for Following Cells and Genes
- Neural Induction: The Default Model
- Neurulation and Neural Tube Defects
- Head to Tail: Hox Genes, Retinoic Acid and Rhombomeres
- Local Organizers: The Midbrain-Hindbrain Boundary
- Ventral to Dorsal: Sonic Hedgehog and BMP in the Neural Tube
- Transcription Factor Codes: Building a Motor Neuron
- Mapping the Cortex: Areas and Arealization
- The Ventricular Zone: Radial Glia as Stem Cells
- Proneural Genes and Lateral Inhibition
- Asymmetric Division: Numb and the Neuroblast
- Reelin and the Reeler Mouse
- Tangential Migration: Interneurons and the Long Way Round
- Crossing the Midline Once: Slit, Robo and Comm
- Semaphorins and Repulsion
- Topographic Maps: Sperry's Eye and Eph Gradients
- Choosing a Partner: Layers, Self-Avoidance and Smell
- The Neurotrophic Hypothesis Tested
- Neurotrophins, Trk Receptors and p75
- The Death Machinery in Neurons
- Competition: How a Target Picks Its Neurons
- The Signal from the Target Travels Home
- Pruning Without Dying: Axons That Cut Themselves Back
- Building the Neuromuscular Junction: Agrin, Lrp4 and MuSK
- Synapses in the Brain: Adhesion, Scaffolds and Astrocytes
- Activity Before Experience: Spontaneous Retinal Waves
- Opening and Closing a Critical Period
- Microglia and Complement Prune Synapses
- Where Glia Come From
- Myelination: Wrapping the Axon
- Feeding the Axon: Glia as Metabolic Partners
- Injury and Regeneration: Why the Brain Does Not Regrow
- When Building Goes Wrong: Microcephaly, Zika and Lissencephaly
- Disorders of the Synapse: Fragile X, Rett Syndrome and Autism
Sensory Systems
- Piezo Channels and the Physics of Force
- Sensory Neurons by Their Genes: the Atlas Era
- Hot, Warm, Cool and Cold
- Nociceptors and the Sodium Channels of Pain
- Itch: A Sense of Its Own
- Sensing the Gut: Enteroendocrine Cells and Nutrient Signals
- Melanopsin Cells: Seeing Without Images
- Photoreceptors Across a Billion-Fold Range, and When They Fail
- The Hair Cell's Transduction Channel
- The Cochlear Amplifier and Otoacoustic Emissions
- Hidden Hearing Loss, Gene Therapy and Regeneration
- One Receptor per Neuron: How Olfactory Neurons Choose
- Taste from Tongue to Cortex