Methods library · Genes and genomes
CRISPR-Cas9 genome editing
Also called Cas9, CRISPR screen, CRISPRi
Cutting a chosen DNA sequence with a guide RNA and Cas9, then reading what the cell's repair leaves behind.
Watch A Cut Is Not a Knockout · 2:46
A schematic drawn in code, narrated by an AI narrator voice (Gemini, Charon) reading narration written from the lesson's text. First made for the lesson Forward and Reverse Genetics: Screens, RNAi and CRISPR.
Where it is taught
12 lessons use this method.