Methods library · Genes and genomes
Cre-lox and binary expression systems
Also called conditional knockout, floxed, Flp-FRT, GAL4-UAS
Deleting or switching on a gene only in chosen cells, or at a chosen time, using a recombinase and its target sites.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
30 lessons use this method.
Developmental Neuroscience
- Tools for Following Cells and Genes
- Mapping the Cortex: Areas and Arealization
- Asymmetric Division: Numb and the Neuroblast
- Tangential Migration: Interneurons and the Long Way Round
- Netrin and DCC: One Cue, Two Answers
- Where Glia Come From
- Feeding the Axon: Glia as Metabolic Partners
- Disorders of the Synapse: Fragile X, Rett Syndrome and Autism
Sensory Systems
- Sensory Neurons by Their Genes: the Atlas Era
- The Low-Threshold Mechanoreceptors
- Merkel Cells: Touch Receptors That Are Not Neurons
- Meissner and Pacinian Corpuscles: Flutter and Vibration
- Hairy Skin, Stroking and Affective Touch
- Where Touch Is First Processed: Dorsal Horn and Dorsal Column Nuclei
- Touch Over-reactivity in Autism Models
- Nociceptors and the Sodium Channels of Pain
- Spinal Circuits That Gate Pain
- Itch: A Sense of Its Own
- Proprioception: Sensing the Body's Own Position
- Vagal Sensory Neurons: The Body Reports to the Brain
- Melanopsin Cells: Seeing Without Images
- Hidden Hearing Loss, Gene Therapy and Regeneration
- One Receptor per Neuron: How Olfactory Neurons Choose