Methods library · Neurons and physiology
Pharmacological blockade and inhibitors
Also called inhibitors, TTX, muscimol, toxins as probes
Testing what a channel, receptor or enzyme does by blocking it with drugs and toxins, with controls for specificity.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
58 lessons use this method.
Cell Signaling
- GTPase Switches: The Timer
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- Lipids as Anchors and Addresses
- GPCRs and Trimeric G Proteins
- PLC, IP3, DAG and Protein Kinase C
- mTOR and AMPK: Reading Nutrients and Energy
- Cytokine Receptors and JAK–STAT
- Notch: Signaling by Contact and by Cutting
- Hedgehog and the Primary Cilium
- Innate Immunity: Toll-like Receptors and NF-κB
- Ion Channels as Receptors
- Signaling to the Nucleus: CREB, AP-1 and Immediate Early Genes
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- The Shape of a Signal: Size, Duration, Frequency and Place
Developmental Neuroscience
- The Wiring Problem: How a Brain Gets Built
- Harrison's Living Axon and the Growth Cone
- Head to Tail: Hox Genes, Retinoic Acid and Rhombomeres
- Ventral to Dorsal: Sonic Hedgehog and BMP in the Neural Tube
- The Ventricular Zone: Radial Glia as Stem Cells
- Radial Migration: Climbing the Glial Ladder
- One Axon, Many Dendrites: Neuronal Polarity
- Too Many Neurons: Naturally Occurring Cell Death
- The Death Machinery in Neurons
- Competition: How a Target Picks Its Neurons
- The Signal from the Target Travels Home
- Pruning Without Dying: Axons That Cut Themselves Back
- Synapse Elimination: One Muscle Fiber, One Axon
- Activity Before Experience: Spontaneous Retinal Waves
- Ocular Dominance and the Critical Period
- Opening and Closing a Critical Period
- Microglia and Complement Prune Synapses
- Sensitive Periods in Behavior: Song, Language and Sight
- Injury and Regeneration: Why the Brain Does Not Regrow
- What Makes the Human Brain Big