Methods library · Cells and imaging
Immunohistochemistry and immunofluorescence
Also called IHC, immunostaining, immunocytochemistry
Showing where a protein sits in cells and tissue by staining with labelled antibodies.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
35 lessons use this method.
Developmental Neuroscience
- Tools for Following Cells and Genes
- Ventral to Dorsal: Sonic Hedgehog and BMP in the Neural Tube
- Transcription Factor Codes: Building a Motor Neuron
- The Ventricular Zone: Radial Glia as Stem Cells
- Asymmetric Division: Numb and the Neuroblast
- Pioneers, Guideposts and the Logic of Guidance Cues
- Crossing the Midline Once: Slit, Robo and Comm
- The Neurotrophic Hypothesis Tested
- Building the Neuromuscular Junction: Agrin, Lrp4 and MuSK
- Synapses in the Brain: Adhesion, Scaffolds and Astrocytes
- Microglia and Complement Prune Synapses
- New Neurons in the Adult Brain
Sensory Systems
- The Low-Threshold Mechanoreceptors
- Where Touch Is First Processed: Dorsal Horn and Dorsal Column Nuclei
- Touch Over-reactivity in Autism Models
- Spinal Circuits That Gate Pain
- Proprioception: Sensing the Body's Own Position
- Melanopsin Cells: Seeing Without Images
- The Hair Cell's Transduction Channel
- Hidden Hearing Loss, Gene Therapy and Regeneration