Methods library · Cells and imaging

Autophagic flux assay

Also called LC3-II, bafilomycin

Measuring autophagy as flux by blocking lysosomal degradation and counting the extra LC3-II that accumulates.

Watch Count the Flux, Not the Dots · 2:52

A schematic drawn in code, narrated by an AI narrator voice (Gemini, Charon) reading narration written from the lesson's text. First made for the lesson Protein Quality Control and Organelle Turnover.

Where it is taught

One lesson uses this method.