Methods library · Proteins and molecules
Engineered mutants: site-directed, dominant-negative and constitutively active
Also called point mutants, domain swap, phosphomimetic, Dominant-negative and constitutively active mutants
Changing chosen residues or domains of a protein, or making mutants that jam or force a pathway, to test what each part does.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
33 lessons use this method.
Cell Signaling
- Phosphorylation: Kinases and Phosphatases
- GTPase Switches: The Timer
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- Lipids as Anchors and Addresses
- PLC, IP3, DAG and Protein Kinase C
- Receptor Tyrosine Kinases
- Ras to MAPK: The Kinase Cascade
- PI 3-Kinase, Akt and Insulin
- TGF-β and Smads
- Wnt: β-Catenin and Planar Polarity
- Notch: Signaling by Contact and by Cutting
- Hedgehog and the Primary Cilium
- Integrins, Rho GTPases and Cell Movement
- Innate Immunity: Toll-like Receptors and NF-κB
- Antigen Receptors and Checkpoints
- Signaling to the Nucleus: CREB, AP-1 and Immediate Early Genes
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Endocytosis and Signaling Endosomes
- How Signaling Evolved
- Oncogenes: When the Switch Sticks
- How We Know: Detecting a Signal