Methods library · Proteins and molecules
Gel electrophoresis, SDS-PAGE and western blot
Also called immunoblot, Gel electrophoresis and SDS-PAGE, PAGE, 2D gel
Detecting one protein, or one phosphorylated form of it, in a whole-cell extract with an antibody after sorting by size.
Watch Sort by Size, Then Ask an Antibody · 1:58
A schematic drawn in code, narrated by an AI narrator voice (Gemini, Charon) reading narration written from the lesson's text. First made for the lesson How We Know: Detecting a Signal.
Where it is taught
22 lessons use this method.
Cell Signaling
- Circuits: Amplification, Feedback, Adaptation and Switches
- Phosphorylation: Kinases and Phosphatases
- mTOR and AMPK: Reading Nutrients and Energy
- Cytokine Receptors and JAK–STAT
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Exosomes and Extracellular Vesicles
- How We Know: Detecting a Signal