Methods library · Proteins and molecules
Protein purification and column chromatography
Also called salting out, gel filtration, ion exchange, affinity chromatography
How to pull one protein out of thousands by solubility, size, charge and affinity, tracking it by its activity.
Watch Purify it, then prove it · 2:51
A schematic drawn in code, narrated by an AI narrator voice (Gemini, Charon) reading narration written from the lesson's text. First made for the lesson Techniques in Protein Biochemistry: Purify It, Then Prove It.
Where it is taught
41 lessons use this method.
Biochemistry
- Amino Acids: How One Side Chain Decides Everything
- Techniques in Protein Biochemistry: Purify It, Then Prove It
- Digestion: Turning a Meal into Cellular Biomolecules
- The Calvin Cycle: Fixing Carbon from Thin Air
- The Pentose Phosphate Pathway: NADPH and Five-Carbon Sugars
- Protein Turnover and Amino Acid Catabolism: Tag, Recognize, Destroy, Recycle
Cell Signaling
- Finding a Signal: NGF, EGF and the Bioassay
- A Field Guide to Receptors
- Phosphorylation: Kinases and Phosphatases
- GTPase Switches: The Timer
- Ubiquitin and Regulated Proteolysis as Signals
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- Lipids as Anchors and Addresses
- cAMP and Protein Kinase A
- PLC, IP3, DAG and Protein Kinase C
- Turning the Signal Off: Arrestins, RGS Proteins and Phosphodiesterases
- Ras to MAPK: The Kinase Cascade
- PI 3-Kinase, Akt and Insulin
- mTOR and AMPK: Reading Nutrients and Energy
- TGF-β and Smads
- Innate Immunity: Toll-like Receptors and NF-κB
- Nuclear Receptors: Receptors That Are Transcription Factors
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Death Receptors, BCL-2 and Caspases
- Endocytosis and Signaling Endosomes
- Hacking the Cell: Reporters, Actuators and Genetic Tools