Methods library · Proteins and molecules
Spectrophotometry and enzyme kinetics
Also called Michaelis-Menten, Lineweaver-Burk, initial velocity, Spectrophotometry and absorption spectra
Measuring concentrations and reaction rates from light absorbance, and turning initial rates into Km, Vmax and inhibition type.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
21 lessons use this method.
Biochemistry
- Amino Acids: How One Side Chain Decides Everything
- Techniques in Protein Biochemistry: Purify It, Then Prove It
- Basic Concepts of Enzyme Action
- Enzyme Kinetics: The Michaelis–Menten Model
- Carbohydrates: One Hydroxyl Decides Storage or Structure
- Digestion: Turning a Meal into Cellular Biomolecules
- Glycolysis: The Investment-and-Payoff Pathway
- Gluconeogenesis: Pushing Glucose Back Uphill
- The Pyruvate Dehydrogenase Complex: The One-Way Gate to the Cycle
- Oxidative Phosphorylation: The Electron-Transport Chain
- Photosynthesis: The Light Reactions
- The Calvin Cycle: Fixing Carbon from Thin Air
- The Pentose Phosphate Pathway: NADPH and Five-Carbon Sugars
- Fatty Acid Synthesis: Building the Chain Two Carbons at a Time