Methods library · Proteins and molecules
Cell-free extracts and reconstitution
Also called in vitro reconstitution, egg extract, cell-free translation, depletion and add-back
Rebuilding a cell process in a tube, then removing and adding back parts to find which are necessary and sufficient.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
33 lessons use this method.
Cell Biology
- Protein Sorting into Compartments
- The Nuclear Envelope and Nuclear Transport
- Vesicle Budding, Targeting and Fusion
- The Secretory Pathway
- Cytoskeletal Polymers and Dynamics
- Molecular Motors and Cargo
- Cell-Cycle States and Control Logic
- Checkpoints and Irreversible Transitions
- Mitosis and Chromosome Segregation
- Cell Death and Clearance
Genetics and Molecular Biology
- DNA Replication: Semiconservative Copying and Its Machinery
- DNA Repair and Recombination
- Transcription: From DNA to RNA
- RNA Processing: Caps, Tails and Splicing
- The Genetic Code and Translation
- The lac Operon: How Bacteria Regulate Genes
- Eukaryotic Gene Regulation and Epigenetics
- Forward and Reverse Genetics: Screens, RNAi and CRISPR
Biochemistry
- Vitalism: How Chemistry Captured Life
- Signal-Transduction Pathways: How Cells Compute With Molecular Switches
- Digestion: Turning a Meal into Cellular Biomolecules
- Glycolysis: The Investment-and-Payoff Pathway
- Photosynthesis: The Light Reactions
- Protein Turnover and Amino Acid Catabolism: Tag, Recognize, Destroy, Recycle
Cell Signaling
- GTPase Switches: The Timer
- cAMP and Protein Kinase A
- PLC, IP3, DAG and Protein Kinase C
- Turning the Signal Off: Arrestins, RGS Proteins and Phosphodiesterases
- Innate Immunity: Toll-like Receptors and NF-κB
- Antigen Receptors and Checkpoints
- Death Receptors, BCL-2 and Caspases
- The Shape of a Signal: Size, Duration, Frequency and Place
- Endocytosis and Signaling Endosomes