Methods library · Proteins and molecules
In vitro kinase assay
Also called phosphorylation assay, phosphoamino acid analysis
Showing that a kinase phosphorylates a substrate by incubating them with labelled ATP and detecting the transferred phosphate.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
14 lessons use this method.
Cell Signaling
- Finding a Signal: NGF, EGF and the Bioassay
- A Field Guide to Receptors
- Phosphorylation: Kinases and Phosphatases
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- PLC, IP3, DAG and Protein Kinase C
- Calcium: The Messenger Kept at Arm's Length
- Turning the Signal Off: Arrestins, RGS Proteins and Phosphodiesterases
- Receptor Tyrosine Kinases
- Ras to MAPK: The Kinase Cascade
- PI 3-Kinase, Akt and Insulin
- How Signaling Evolved
- Oncogenes: When the Switch Sticks
- Drugs Against a Part
- How We Know: Detecting a Signal