Methods library · Proteins and molecules
Binding assays: immunoprecipitation, pull-down and gel shift
Also called co-IP, pull-down, yeast two-hybrid, EMSA and DNA footprinting
Testing whether two molecules bind by pulling one down and looking for the other, or by slowed migration in a gel.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
24 lessons use this method.
Cell Signaling
- Phosphorylation: Kinases and Phosphatases
- Ubiquitin and Regulated Proteolysis as Signals
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- Adaptors, Docking Proteins and Scaffolds
- PI 3-Kinase, Akt and Insulin
- Cytokine Receptors and JAK–STAT
- Innate Immunity: Toll-like Receptors and NF-κB
- Antigen Receptors and Checkpoints
- Death Receptors, BCL-2 and Caspases
- Reverse Signaling: When the Ligand Is Also a Receptor
- How We Know: Detecting a Signal
Developmental Neuroscience
- Neural Induction: The Default Model
- Transcription Factor Codes: Building a Motor Neuron
- Reelin and the Reeler Mouse
- Netrin and DCC: One Cue, Two Answers
- Topographic Maps: Sperry's Eye and Eph Gradients
- Choosing a Partner: Layers, Self-Avoidance and Smell
- Building the Neuromuscular Junction: Agrin, Lrp4 and MuSK