Methods library · Genes and genomes
Forward genetic screens
Also called mutagenesis screen, enhancer screen, EMS screen
Finding the genes for a process by mutagenizing at random and picking out the mutants that break it.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
35 lessons use this method.
Genetics and Molecular Biology
- Complementation and Gene Function: From Beadle and Tatum to Benzer
- Bacterial and Phage Genetics: Conjugation, Transduction and Transformation
- DNA Replication: Semiconservative Copying and Its Machinery
- Mutation: Where Variation Comes From
- Eukaryotic Gene Regulation and Epigenetics
- Forward and Reverse Genetics: Screens, RNAi and CRISPR
Cell Signaling
- Ubiquitin and Regulated Proteolysis as Signals
- Adaptors, Docking Proteins and Scaffolds
- mTOR and AMPK: Reading Nutrients and Energy
- Cytokine Receptors and JAK–STAT
- TGF-β and Smads
- Wnt: β-Catenin and Planar Polarity
- Notch: Signaling by Contact and by Cutting
- Hedgehog and the Primary Cilium
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Death Receptors, BCL-2 and Caspases
Developmental Neuroscience
- The Wiring Problem: How a Brain Gets Built
- Head to Tail: Hox Genes, Retinoic Acid and Rhombomeres
- Proneural Genes and Lateral Inhibition
- Asymmetric Division: Numb and the Neuroblast
- Birth Order and Fate: Temporal Identity and the Inside-Out Cortex
- Pioneers, Guideposts and the Logic of Guidance Cues
- Netrin and DCC: One Cue, Two Answers
- Crossing the Midline Once: Slit, Robo and Comm
- The Death Machinery in Neurons
- Injury and Regeneration: Why the Brain Does Not Regrow