Methods library · Genes and genomes
Gene cloning: vectors, libraries and screening
Also called restriction enzymes, plasmid vector, transformation, Gene cloning by library screening
Cutting and joining DNA into vectors, growing it in bacteria, and fishing a gene out of a cDNA or genomic library.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
29 lessons use this method.
Cell Signaling
- A Field Guide to Receptors
- Protein Modules: SH2, SH3, PTB, PDZ and PH
- Adaptors, Docking Proteins and Scaffolds
- GPCRs and Trimeric G Proteins
- Turning the Signal Off: Arrestins, RGS Proteins and Phosphodiesterases
- Sensing the World: Vision, Smell, Nitric Oxide and cGMP
- Receptor Tyrosine Kinases
- PI 3-Kinase, Akt and Insulin
- TGF-β and Smads
- Wnt: β-Catenin and Planar Polarity
- Notch: Signaling by Contact and by Cutting
- Integrins, Rho GTPases and Cell Movement
- Innate Immunity: Toll-like Receptors and NF-κB
- Antigen Receptors and Checkpoints
- Nuclear Receptors: Receptors That Are Transcription Factors
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- Death Receptors, BCL-2 and Caspases
- The Shape of a Signal: Size, Duration, Frequency and Place
- Reverse Signaling: When the Ligand Is Also a Receptor
- Hacking the Cell: Reporters, Actuators and Genetic Tools