Methods library · Cells and imaging
Fluorescent protein tags and biosensors
Also called GFP fusion, fluorescent protein, Genetically encoded biosensors (FRET), FRET sensor
Fusing GFP and engineered fluorescent sensors to proteins to watch where they go and what signals they report in living cells.
The film for this method is in production. Until then, the lessons below explain it where they use it.
Where it is taught
19 lessons use this method.
Cell Signaling
- Lipids as Anchors and Addresses
- cAMP and Protein Kinase A
- Calcium: The Messenger Kept at Arm's Length
- PI 3-Kinase, Akt and Insulin
- Hedgehog and the Primary Cilium
- Stress Signals: p38 and JNK, p53, Unfolded Proteins and Oxidants
- The Shape of a Signal: Size, Duration, Frequency and Place
- Endocytosis and Signaling Endosomes
- Long-Distance Signaling in Neurons
- Hacking the Cell: Reporters, Actuators and Genetic Tools