Methods library · Genes and genomes

PCR and quantitative PCR

Also called qPCR, RT-qPCR, polymerase chain reaction

Copying one stretch of DNA billions of times with primers and a heat-stable polymerase, and counting cycles to quantify it.

Watch Copies of Copies, Doubling Each Cycle · 2:37

A schematic drawn in code, narrated by an AI narrator voice (Gemini, Charon) reading narration written from the lesson's text. First made for the lesson Recombinant DNA, Cloning and PCR.

Where it is taught

8 lessons use this method.